Agent-Based Model of Therapeutic Adipose-Derived Stromal Cell Trafficking during Ischemia Predicts Ability To Roll on P-Selectin
Figure 10
Simulated microvascular network was modeled after mouse skeletal muscle, visualized using confocal microscopy following harvest, using a 20× objective.
(A) Confocal microscopy image of mouse spinotrapezius muscle immuno-stained to visualize ECs with BS1-lectin antibody (white). Vascular structures of interest were copied over in yellow in image processing software (ImageJ). Scale bar is 1 mm. (B) The micrograph was manually discretized into nodes, defined as bifurcation points in the microvascular network, and the nodes were connected to form elements. (C) Screen-shot of simulation space. Nodes and elements were manually drawn into the NetLogo simulation space to represent the real microvascular network. Arterioles and venules were characterized on the micrograph based on vessel diameter. Smooth muscle cells are depicted in red lining arterioles and venules. Endothelial cells are depicted in yellow, and tissue macrophages present within the interstitum of the simulation space are depicted in blue.