An Analysis of Enzyme Kinetics Data for Mitochondrial DNA Strand Termination by Nucleoside Reverse Transcription Inhibitors
Figure 3
The relationship between the IC50 values and the specificity constant, kcat/Km.
The specificity constant is a measurement of direct interaction with polymerase-γ often used as prediction for analog incorporation. The IC50 values are a more direct measure of incorporation probability taking exonuclease activity and other features into account. This relationship shows that the specificity constant is a useful proxy for incorporation probability.