Distinct Modes of Regulation by Chromatin Encoded through Nucleosome Positioning Signals
Figure 14
Deletion of a Poly(dA:dT) element from a replication origin results in a reduction in replication efficiency.
(A) Shown is the average (predicted) nucleosome occupancy of the nucleosome positioning model that we developed here (red) at the 6 kb region surrounding the one replication origin from S. pombe (“ARS 3002”) that was studied in the systematic sequence deletion study of [60]. Our model predicts a nucleosome depleted region around the replication origin (“ARS 3002”). Annotated replication origins in the region were taken from [60] (B) Same as (A), but only around the 815 bp region of the studied origin (“ARS 3002”). (C) Schematic representation of the 15 regions of length ∼50 bp that were each deleted in the study of [60]. The replication efficiency of each of these 15 regions was tested in [60], and it was found that of all 15 regions, deletion of region 10 (which contains a Poly(dA:dT) element) resulted in the largest reduction in replication efficiency. (D) The DNA sequence of region 10 from [60]. The Poly(dA:dT) element is indicated.