Inference of Protein Complex Activities from Chemical-Genetic Profile and Its Applications: Predicting Drug-Target Pathways
Figure 4
Significantly sensitive protein complexes to drugs known for their target pathway.
The significance score of the effect of a compound on a protein complex was estimated using error function (see Method for details). When a significance score of a compound on a complex was less than a given threshold, and also that complex has relatively positive value of an activity, the complex was defined as the “sensitive complex” to that compound. For rapamycin in cluster I, there is only one sensitive complex, PC 321. There are four sensitive complexes, PC 379, PC 413, PC 148, and PC 366 to compounds in cluster II which are microtubule-poisons. Those DNA-damaging agents of cluster III have more than five sensitive complexes, PC 181, PC 170, PC 65, PC 290, and PC 424. The types of biological associations between each sensitive complex and their sensitive strains are available, and also GO analysis results of the set of those genes of such sensitive strains at http://pombe.kaist.ac.kr/CMA/ModeOfAction.pl.