Inference of Protein Complex Activities from Chemical-Genetic Profile and Its Applications: Predicting Drug-Target Pathways
Figure 3
Clustering analysis of protein complex activities.
(A) Bird eyes of two-dimensional hierarchical clustering analysis of protein complex activities (488 PCs by 82 drugs). (B) The PC-based hierarchical dendrogram of 82 drugs using relative activities of all of 488 protein complexes. The red star and red vertical bar indicates the group of drugs very closely clustered in the PC-based clustering but not in strain-based clustering using the same compendium [5]. The blue star and blue vertical bar represent the group of drugs very closely clustered in both clustering. The rectangular dashed line box shows the group of drugs clustered slightly differently in each clustering. The alphabets in parenthesis denote the groups of drugs with similar mode-of-action supported by literatures. (C) The strain-based hierarchical dendrogram of 82 drugs using the growth fitness of 3,418 strains with at least one measurement above Log2 ratio 0.5 as performed in the compendium paper [5]. (D) The examples of the clusters of PCs in similar biological functions. We selected three clusters which have at least two PCs with known function. The annotation in the right denotes “complex ID: complex name: complex localization”.