Allosteric Communication in Myosin V: From Small Conformational Changes to Large Directed Movements
Figure 7
Lever-arm motion encoded in the lowest-frequency modes in the rigor-like and post-rigor states of myosin V.
(A) Pictorial representation of the lever oscillation along modes 1 (red) and 3 (blue) in the rigor-like and post-rigor states. (B) Lever-arm motion reported in spherical coordinates along the lowest-frequency modes. The spherical coordinates φ and θ, which correspond to the zenith and the azimuth angle, respectively, were determined by fitting the coordinates of the lever-arm backbone atoms (aa 754–792) upon optimal superposition of the N, U50, and L50 subdomains to the equilibrium structure. The circle and square correspond to the orientation of the lever arm in the rigor-like and post-rigor conformations, respectively. Mode 1 and 3 are essentially perpendicular in both functional states.