The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging
Figure 10
The assessment of the accuracy of the diffusion model for the Lyn-Src, KRas-Src, and Cytosolic-Src biosensors.
(A) the difference (absolute value) of fluorescence intensity between simulated and experimental images for (i) Lyn-Src biosensor at 10 sec after photobleaching, (ii) KRas-Src biosensor at 10 sec after photobleaching, (iii) Cytosolic-Src biosensor at 1 sec after photobleaching because of its fast recovery rate; (B, i–iii) the scattered plots of smoothed WDLC vs. smoothed WCCT for the corresponding cells shown in (A, i–iii).