The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging
Figure 9
The experimental FRAP images for the cytosolic Src biosensor are compared with those predicted by simulation.
(A) Left: the fluorescence intensity image of a cell before photobleaching, with the red-colored outline defining the cell edge in simulation and blue-colored outline defining the region of interest monitored for fluorescence recovery. Middle and right: the fluorescence intensity images at 0 and 1 min after photobleaching, respectively. (B) The time course of fluorescence recovery in the photobleached area as marked in (A). (C) Left: the concentration map after photobleaching (0 sec), computed by normalizing the fluorescence intensity with the image before photobleaching. Middle and right: the experimental and simulated concentration maps at 6 seconds after photobleaching, with the difference between experiment and simulate indicated by two arrows. (D) The scattered plot of the weighted Laplacian of the concentration vs. the weighted change of concentration in time on each mesh node, with the linear fitting indicated by the solid line.