The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging
Figure 8
The experimental recovery images after photobleaching of Src biosensor targeted to plasma membrane outside of lipid rafts (KRas-Src biosensor) are compared with those predicted by simulation.
(A) Left: the fluorescence intensity image of a cell before photobleaching, with the red-colored outline defining the cell edge in simulation and blue-colored outline defining the region of interest monitored for fluorescence recovery. Middle and right: the fluorescence intensity images at 0 and 1 min after photobleaching, respectively. (B) The time course of fluorescence recovery in the photobleached area as marked in (A). (C) Left: the concentration map after photobleaching (0 min), computed by normalizing the fluorescence intensity with the reference image before photobleaching. Middle and right: at 1 min after photobleaching, the experimental concentration map is similar to the simulation. (D) The scattered plot of the weighted Laplacian of the concentration vs. the weighted change of concentration in time on each mesh node, with the linear fitting indicated by the solid line.