The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging
Figure 7
The experimental FRAP images of Lyn-Src biosensor are compared with those predicted by simulation.
(A) Left: the fluorescence intensity image of a cell before photobleaching, with the red-colored outline defining the cell edge in simulation and blue-colored outline defining the region of interest monitored for fluorescence recovery. Middle and right: the fluorescence intensity images at 0 and 1 min after photobleaching, respectively. The complete time course of this FRAP experiment is shown in Movie S1. (B) The time course of fluorescence recovery in the photobleached area as marked in (A). (C) Left: the concentration map after photobleaching (0 min), computed by normalizing the fluorescence intensity with the image before photobleaching. Middle and right: the experimental and simulated concentration maps at 1 min after photobleaching. (D) The scattered plot of WDLC vs. WCCT on each mesh node, with the linear fitting indicated by the solid line.