The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging
Figure 6
The FE analysis procedures for assessing and simulating diffusion based on experimental FRAP images are illustrated.
With two consecutive experimental concentration maps and the time interval (dt), the apparent diffusion coefficient and boundary conditions were estimated by using our FE-based diffusion analysis. Subsequently, the diffusion coefficient and the boundary conditions were used to simulate and predict the concentration image at the next time step (est_un+1), which was produced by allowing linear diffusion from the current image (un). The main outputs of the described procedure are the apparent diffusion coefficient and the simulated concentration maps, as outlined in the dashed boxes.