The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging
Figure 5
The characterization of the Src biosensors.
(A) The Lyn-Src biosensor is anchored to the lipid rafts of the plasma membrane via N-terminal acylation sequences derived from the N-termini of Lyn kinase; the KRas-Src biosensor is connected to the non-lipid-rafts region through C-terminal prenylation sequences derived from KRas. Panel (B) shows the expression level of HeLa cells transfected with Cytosolic-Src, Lyn-Src, or KRas-Src biosensors, from left to right, respectively.