The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging
Figure 4
The validation of the FE-based method using computer simulation.
(A) The initial concentration map of a diffusive fluorescence molecule in a single cell; (B) the concentration map at 0.0313 s produced by computer simulation with an assigned diffusion coefficient of 29 µm2/sec; (C) the simulated fluorescence recovery curve for 9 sec after photobleaching; (D) the scattered plot of the WDLC, −dt·K·0.5(un+un+1), vs. the WCCT, M(un+1−un), on each mesh node. Linear fitting is represented by the solid line.