The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging
Figure 3
The computational procedure used to simulate FRAP experiment and calculate diffusion coefficient to evaluate the accuracy of the FE-analysis.
With an assigned cell geometry, an initial concentration, and a diffusion coefficient, a series of concentration images at later time steps were generated using the finite element method and diffusion model. The simulated concentration maps were then used as the input to calculate diffusion coefficients using FE analysis and linear regression. This calculated diffusion coefficient was compared with the assigned diffusion coefficient to examine the accuracy of the method. The main output of this procedure is the simulated concentration images and the estimated diffusion coefficient as outlined in the dashed boxes. The data connected by dashed line arrows were shared between different layers.