The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging
Figure 2
The flow chart representing the application of FE method to discretize the cell geometry and calculate diffusion coefficient using Fick's Law and linear regression based on two consecutive concentration images and the time interval (dt).
Step (A) shows the computation of stiffness matrix K and the mass matrix M based on the FE discretization on the cell geometry. In step (B), the concentration vectors (un and un+1) are obtained by collecting the concentration values at the nodes of FE discretization in the images. In step (C), the least-square linear fitting is used to estimate the diffusion coefficient based on all the information from the previous steps: M, K, un, un+1 and dt.