The Spatiotemporal Pattern of Src Activation at Lipid Rafts Revealed by Diffusion-Corrected FRET Imaging
Figure 1
A schematic illustration on using FE analysis to compute the kinase activity map from apparent FRET video images.
(A) An image showing the original location of activated FRET biosensors. (B) A possible apparent FRET signals at the next time step, where newly activated biosensors (hence actions of kinase) are mixed with those translocated from (A) due to diffusion. (C) The simulated FRET distribution map of the biosensor due to diffusion from (A). (D) The actions of kinase activity detected by subtracting (C) from (B). Yellow or red dots represent the FRET biosensors activated by the target kinase at different time steps. Green dots represent the activated biosensors translocated from other locations due to diffusion.