Stereochemical Criteria for Prediction of the Effects of Proline Mutations on Protein Stability
Figure 3
The flowchart of scheme for prediction of effects of Pro mutations on protein stability derived from structural analysis of WT crystal structure and mutant models. A and I refer to active and inactive predictions, respectively. Letters in lowercase (a–f) refer to each node of the scheme as described in the text. Numbers at each branch indicate the number of active and inactive mutants satisfying the “Yes” or “No” criteria of the respective branch. The numbers at each A and I prediction bubble correspond to the number of CcdB Pro mutants ending at that bubble. The numbers of misclassified mutants are shown in italics. Active mutants correspond to nonperturbing Pro substitutions and inactive ones to perturbing Pro substitutions in case of CcdB.