Stereochemical Criteria for Prediction of the Effects of Proline Mutations on Protein Stability
Figure 2
Correlation of Pro Mutant Activity with Various Structural Parameters of WT and Mutant Models of CcdB
Solid circles represent active mutants and empty circles represent inactive mutants. (A) WT residue ACC, (B) WT residue depth, (C) |φ(WT) − (−65)| (D) solubility of mutant (0 denotes insoluble and 1 denotes soluble), (E) number of H-bonds formed by WT residue in native structure and acceptor in mutant models (−1 denotes WT amide not involved in H-bond, 0 denotes WT amide involved in H-bond but acceptor of this amide group not satisfied in any of the mutant models and 1 denotes acceptor forms new H-bond in at least one mutant model). Correlations with p-values less than 0.05 are considered statistically significant.