A Nucleosome-Guided Map of Transcription Factor Binding Sites in Yeast
Figure 6
Transcriptional Complexes Involving Ste12, Tec1, and Dig1
(A) During filamentation, Ste12 forms a complex with Dig1 and Tec1. Tec1 binds DNA, with a sequence specificity for CATTCy. PRIORITY-D℘N℘ finds this motif in all three sequence-sets pulled down by Ste12, Tec1, and Dig1 after the cells are treated with butanol. However, PRIORITY-U℘ misses the functional Tec1 motif in Ste12_BUT14 and Dig1_BUT14. The asterisk indicates that the learned motif is a weak match.
(B) During mating, Ste12 forms two complexes: one with Dig1 and Dig2, and another with Dig1 and Tec1. In either case, it is Ste12 that binds DNA, with a sequence specificity for ATGAAAC. Again, PRIORITY-D℘N℘ finds this motif in all three sequence-sets pulled down by Ste12, Tec1, and Dig1 after the cells are treated with the alpha factor pheromone. Here, PRIORITY-U℘ fails to find the Ste12 motif in Tec1_Alpha. (Figures of the complexes are adapted from Chou et al. [25].)