Self-organizing Mechanism for Development of Space-filling Neuronal Dendrites
Figure 9
Procedures for Quantification of DOB
Step 1: Skeletonize images with ImageJ. Step 2: Crop four pairs of squares. Step 3: Approximate each dendritic segment between the two branching points by a line segment connecting those points. Measure the angle of a branch segment with respect to the horizontal direction. Repeat measurement for all segments in each local area and calculate the coefficient of variation, DOB.