A Stochastic View of Spliceosome Assembly and Recycling in the Nucleus
Figure 6
Expression of SPN1ΔN Affects the Mobility of Splicing Proteins in Living Cells
FLIP experiments were performed on cells expressing GFP-U2AF65 (A) and GFP-SmE (B) together with the wt (+wt SPN1, red curves) or the dominant-negative mutant (+SPN1ΔN, green curves) forms of snurportin1. The fluorescence decay was analyzed over nuclear speckles. The results obtained for GFP-U2AF65 and GFP-SmE in cells that were not transfected with any SPN1 construct are also shown (black curves). Each decay curve corresponds to a pool of three independent experiments, with ten different cells analyzed per experiment.