A Stochastic View of Spliceosome Assembly and Recycling in the Nucleus
Figure 4
A Deletion Variant of Snurportin 1 Affects the Subnuclear Distribution of snRNPs
HeLa cells were transfected with either wt snurportin1 (wt SPN1) (A–D) or the deletion variants SPN1ΔN (E–H), and SPN1ΔC (I–L) fused to GFP. The cells were labeled with mAb Y12 directed against Sm proteins.
(A,E,I) Depict the superimposition of red (Y12 antibody staining) and green (GFP-tagged protein) images.
(B,C,F,G,J,K) Depict the same cell imaged for GFP-tagged protein and Y12 staining, as indicated; arrows point to CBs and arrowheads to nuclear speckles. Bar indicates 10 μm. (D,H,L) Show the western blot analysis of each fusion protein. Molecular weight markers (kDa) are indicated on the left.