Metabolic Reconstruction and Modeling of Nitrogen Fixation in Rhizobium etli
Figure 3
Experimental Validation with In Silico Gene Deletions
The effects that mutations have on symbiotic nitrogen fixation was evaluated using linear (FBA) and quadratic (MOMA) programming [35]. This table shows the predicted increases (+) or decreases (−) in the OF, nitrogenase flux, PHB flux, and glycogen synthase flux with respect to the wild-type strain. Each entry of the table contains a pair of signs representing the effects of the deleted reaction on the fluxes. The first sign is the effect predicted by FBA, the second is that obtained from MOMA. At the end of each row we report the references that give experimental support to the increase or decrease of nitrogen fixation after the corresponding gene deletion. A brief description of the experimental assessment is presented in each gene deletion. In silico prediction of the effects of the double gene deletion (PHB and glycogen) depends on the optimization method (FBA or MOMA).