Design of Multi-Specificity in Protein Interfaces
Figure 1
Computational Strategy and Methodology Flowchart
(A) Computational strategy for determining the degree of optimization and predicted cost of multi-specificity.
(B) Flowchart illustrating the methodology for generating a dataset of multi-specific proteins and computational protocol for predicting sequences optimal for each binding interaction alone (single-constraint) as well as sequences predicted to satisfying binding in the context of all structurally characterized partners (multi-constraint).