Image-Based Modeling Reveals Dynamic Redistribution of DNA Damage into Nuclear Sub-Domains
Figure 10
No Decondensation of Chromatin following 5 Gy of Low LET
HeLa cells transfected with H1.2-GFP (see Materials and Methods) were imaged before and after exposure to 5 Gy of X-rays (A). Control cells that were not exposed to X-rays were also monitored (B). Intensity correlation within the nucleus (contour shown in blue on images) was computed between consecutive time points. To correct for noise which is unique to each image, correlation values were normalized to the average correlation measured for the three first time points (i.e., before radiation). These relative correlations are graphed in (C) for both groups (i.e., each group represents averages from five different nuclei). Correlation values before and after radiation (20–50 min) are shown on the graph by dashes on the bars for both control and irradiated specimen.