Image-Based Modeling Reveals Dynamic Redistribution of DNA Damage into Nuclear Sub-Domains
Figure 4
Comparing Theoretical and Experimental DNA Damage Pattern along 1 GeV/amu Fe Track
Average distributions of distances between consecutive foci along Fe tracks are plotted at 4.5 min and 35 min following 1 Gy exposure (blue solid lines, (A) and (B), respectively). Error bars are standard errors based on four independent experiments. For each individual track analyzed from real data, foci were counted and their positions were then randomized based on DNA profiles, described previously, to generate a theoretical distribution pattern (red dashed lines). Measuring the correlation between theoretical and experimental distributions, we observe a decrease of correlation between these two time points, from 0.6 to 0.45. These data indicate that as early as 4.5 min following exposure to radiation, foci positions deviate from a theoretical random behavior by 40% and this tendency increases over the next 30 min with a 55% loss of correlation.