Image-Based Modeling Reveals Dynamic Redistribution of DNA Damage into Nuclear Sub-Domains
Figure 1
Comparison between Simulated and Experimental Images for Different Types of Radiation
(A) Shows RIF within a nucleus traversed by high energy Fe ions (1 GeV/amu Fe).
(B) shows a nucleus exposed to photons.
Shown at the top are RIF images of nuclei taken from a microscope (DAPI in blue and DNA damage marker γH2AX in green) 5–10 min post-IR. Middle images are pRIF microscopic images at the same magnification. These pRIF images are generated by blurring DNA damage simulations for equivalent doses of radiation with the PSF of the optic used. For pRIFs, the blue channel shows the resulting nuclear density and the green channel shows DSB. If the PSF is omitted from the simulation, DSB locations can be better resolved, as shown in the gray images at the bottom of each panel (brightness proportional to the number of breaks within each pixel). Scaling bar shown in white is 1 μm wide.