Evolution of Function in the “Two Dinucleotide Binding Domains” Flavoproteins
Figure 9
Structural Overlap of Putidaredoxin Reductase and Putidaredoxin with Analogous Redox Pairs from Different Subgroups
(A) Structural superposition was performed using coordinates of putidaredoxin reductase (PDB:1q1w), adrenodoxin reductase (1e6e), and 2,4-dienoylCoA reductase (1ps9). Putidaredoxin was superimposed separately on adrenodoxin from the adrenodoxin reductase/adrenodoxin complex (1e6e). The tDBDFs are shown in cyan and the interacting proteins are shown in the following colors: putidaredoxin (PDB:1oqr), blue; adrenodoxin (PDB:1e6e), gold; and N-terminal extension of 2,4-dienoylCoA reductase (1ps9), forest green.
(B) Zoomed view of the active site in A, showing one-electron acceptors and the FAD cofactor. Trp106 and Met70 from putidaredoxin are shown in blue and Trp330 from putidaredoxin reductase is shown in cyan.