Towards Complete Sets of Farnesylated and Geranylgeranylated Proteins
Figure 7
Western Blots and TLC Scanning Results for FLJ32421 (BROFTI) with Radioactive Prenyl Anchor Precursors
Western blot and corresponding scans from TLC linear analyzer of wild-type GST-FLJ32421-fusion protein translated with [3H]mevalonic acid (lane 1), GST-FLJ32421 C408A with [3H]mevalonic acid (lane 2), GST-FLJ32421 with [3H]FPP (lane 3) and GST-FLJ32421 with [3H]GGPP (lane 4). There is significant incorporation of a product of mevalonic acid as well as FPP, while incorporation of GGPP is close to the detection limit, suggesting that FLJ32421 (BROFTI) is primarily a farnesylation target.