Dynamic Simulations on the Arachidonic Acid Metabolic Network
Figure 5
The Production of 15-HETE in the Presence of Inhibitors
PMNs (1.66 × 107 cells/ml) were preincubated with inhibitors for 10 min at 37 °C and then were stimulated with 10 μM A23187 (a calcium ionophore) for 1 h at 37 °C. MK886, an inhibitor of 5-LOX, was used to block the production of LTs. CAY10404, an inhibitor of COX-2, was used to block the production of PGs. The y-axis represents the production of 15-HETE with exogenous AA normalized against that without exogenous AA.