Dynamic Simulations on the Arachidonic Acid Metabolic Network
Figure 4
Effects of Exogenous AA on the Network Balance
The 1-h output of ω-LTB4 (20-OH-LTB4 + 20-COOH-LTB4) (A) and 15-HETE (B) were calculated to evaluate changes in the 5-LOX pathway and the 15-LOX pathway. To validate the simulation, experiments were performed: PMNs (1.66 × 107 cells/ml) were incubated at 37 °C with 10 μM A23187 (a calcium ionophore) and arachidonic acid of different concentration for 1h. The output of ω-LTB4 (C) and 15-HETE (D) were measured. The y-axis represents the production with exogenous AA normalized against that without exogenous AA.