A Mathematical Model for Neutrophil Gradient Sensing and Polarization
Figure 10
Double Micropipette Experiment
Steady-state distribution of PIP3, actin, and myosin in response to two opposing micropipettes placed equidistantly around the cell. One gradient was fixed and the second was varied in intensity. The relative strength is the ratio of the peak concentrations of the two chemoattractant sources. When the chemoattractant profiles of the micropipettes are equal (relative strength equal to one), the cell forms two fronts and two backs. When one micropipette has a significantly higher peak concentration than the other, only one front and one back forms. The nominal parameters for both gradients is bL = 1 and gL = 50%.