Both Ligand- and Cell-Specific Parameters Control Ligand Agonism in a Kinetic Model of G Protein–Coupled Receptor Signaling

doi:10.1371/journal.pcbi.0030006

Both Ligand- and Cell-Specific Parameters Control Ligand Agonism in a Kinetic Model of G Protein–Coupled Receptor Signaling

Figure 2

Time Course of Representative Model Outputs

Parameter values are sampled using LHS, and the differential equations describing the cTCAM are solved according to the equations in Text S1. G protein activation as quantified by Gα_GTP is tracked over time (see Equation S.27 in Text S1).

(A) Values of Gα_GTP (number/cell) for eight parameter sets from LHS sampling of the ranges in Table 1 are plotted over the course of the simulation.

(B) Percent change in the value of Gα_GTP relative to basal values (%OverBasal) was calculated and tracked over time according to Equation 1. [L] = 0.1 μM.

doi: https://doi.org/10.1371/journal.pcbi.0030006.g002