Detecting Statistically Significant Common Insertion Sites in Retroviral Insertional Mutagenesis Screens
Figure 10
Schematic Depiction of the Computation of a Background-Corrected Threshold
(A) The density of TSSs (the 5′ ends of the genes) is computed using a fixed kernel width hbg.
(B) A new realization of insertions is generated using the density from step A.
(C) The GKC method is applied to the resulting insertion profile, yielding one realization of the background density estimate. Steps (A) and (B) and applying the GKC are repeated N times to yield a distribution of background realizations. For every position on the genome, a CDF of these realizations is computed and the threshold is determined based on the α-level.
(D) The location-dependent threshold is combined with the threshold based on uniform background. Finally, the smoothed insertion estimate of the real data is thresholded with the resulting threshold.