Detecting Statistically Significant Common Insertion Sites in Retroviral Insertional Mutagenesis Screens
Figure 9
Schematic Depiction of the Significance Analysis of the Density Estimate of the Insertion Data
(A) The position of the N insertions is permuted.
(B) The convolution method is applied to the resulting permuted insertion profile. The heights of all peaks are recorded.
(C) Step A and B are repeated. A distribution of the peaks in random data results.
(D) The threshold is computed by determining the α-level in the empirical CDF of the null-distribution. This threshold is applied to the insertion estimate of the real insertion data, resulting in a series of significant peaks.