Detecting Statistically Significant Common Insertion Sites in Retroviral Insertional Mutagenesis Screens
Figure 8
Example of Novel CIS and Background Corrected CIS
(A) Venn diagram comparing the csCISs and the CISs in the RTCGD. For reasons explained in Figure 7, the intersection shows two counts.
(B) An example of a CIS that consists of three insertions from three independent screens, and therefore is only detected when integrally analyzing the data.
(C) Venn diagram comparing the csCISs with and without applying background correction.
(D) An example of a csCIS, that was also included in the RTCGD, and is rejected based on the background-corrected threshold. The small vertical bars (red) in the genes denote the 5′ ends of genes, and a star denotes a corrected CIS. Since we are only interested in correcting regions that are putative CISs, a background-corrected threshold is only computed for peaks in the estimated number of insertions. The corrected threshold is given by the horizontal dotted line above the peak.