Detecting Statistically Significant Common Insertion Sites in Retroviral Insertional Mutagenesis Screens
Figure 3
The Myc Locus on Chromosome 15
(A) The blue line represents the estimated number of insertions as a function of position for a certain region. The red line depicts the threshold associated with an α-level of 0.05.
(B) CISs are depicted by means of vertical lines. From top to bottom these represent: the CISs for the current scale (30k), the csCISs, the CISs from the RTCGD, the insertions, and the genes (top and bottom strand separated).
(C) Scale space diagram. The vertical axes of the scale space has a logarithmic scale and indicates the scale for which the CIS was detected (only a subset of scales was actually evaluated: [50 100 250 500 1 k 2.5 k 5 k 10 k 30 k 50 k 100 k 150 k] bp).
(D) Evaluation of the insertion distribution over four small scale CISs, identified by scale space analysis. Per screen we list the number of insertions that fall within the small scale CIS. The screens are labeled consistent with RTCGD nomenclature.