Detecting Statistically Significant Common Insertion Sites in Retroviral Insertional Mutagenesis Screens
Figure 2
Schematic Depiction of the Kernel Convolution Framework
The insertions are convolved with a kernel function with a width determined by the scale parameter. In principle any kernel function can be used, but the Gaussian kernel function is depicted. The significance of the peaks is evaluated using a null-distribution computed by means of a random permutation of the data. This is done for a range of scale parameters to obtain the CISs in the scale space.