Model-Based Design of Growth-Attenuated Viruses
Figure 4
Relative Expression Rates of Viral Proteins in BHK Cells Infected by Individual VSV Strain
The expression rate of each protein was normalized by that of N protein. Therefore, the relative expression rate of N protein is defined as one. X and Y coordinates of datapoints indicate the results of experiments and simulations, respectively. All the experimental datapoints were obtained from the literature [18]. The four datapoints in the circle denote the relative protein expression rates for the genes located at the second genome position in the cases of MGP, MPG, GPM, and GMP strains having gene orders 3′-N-M-G-P-L-5′, 3′-N-M-P-G-L-5′, 3′-N-G-P-M-L-5′, and 3′-N-G-M-P-L-5′, respectively. PMG and PGM strains have the gene orders 3′-N-P-M-G-L-5′ and 3′-N-P-G-M-L-5′, respectively. The two rectangular boxes include four datapoints where there are the largest discrepancies between simulation and experimental results. Vex(0) = 5.