Wiggle—Predicting Functionally Flexible Regions from Primary Sequence
Figure 8
Wiggle Predictors Identify Regions Corresponding to Glycosylation Sites on Erythropoietin
(A) FF score plotted against residue number with thresholds shown in red. Erythropoietin is modeled by the GNM in the complexed form with the corresponding receptor (not shown). All residues have below mean fluctuation (colored blue), but none of the residues are defined as FFRs since they do not exceed the definition threshold. The four glycosylation sites (S126 and lysine substituted K24, K38, and K83) along with G151 are labeled. (PDB ID: 1EER)
(B) FFRs correspond to positive values as predicted by Wiggle (solid line) and Wiggle200 (dashed line) which are structurally mapped onto erythropoetin (green and orange, respectively). Not all loops are identified by the predictors to be functionally flexible, thus showing that discrimination is not based on structural features.