Wiggle—Predicting Functionally Flexible Regions from Primary Sequence
Figure 2
FF Score Identifies FFR in Bovine Pancreatic Inhibitor and Calmodulin
Comparison of unweighted (dashed line) and weighted (solid line) FF scores for BPTI ([top], PDB ID: 5PTI) and calmodulin (bottom, PDB ID: 1CLL). FF scores are mapped with the same gradient coloring from negative (blue) to positive (red) as the scale shown in Figure 1D. Both recognition loops (loop 1: residues 11 to 19; loop 2: residues 35 to 42) are identified in BPTI by the FF score, whereas loop 2 is not identified with the unweighted mode. For calmodulin, the FF score allows us to identify the central hinge for this protein (residues 68 to 91 shown in blue because it exceeds the negative threshold of less than −1.5). This central helix, containing eight turns, is known to collapse when bound to calcium and substrate.