Key Role of Local Regulation in Chemosensing Revealed by a New Molecular Interaction-Based Modeling Method
Figure 6
Correspondence in Time and Space between the Predicted and Measured Changes in Membrane-Bound PTEN at the Front and Back of the Cells Exposed to cAMP Gradients
These dose-dependent dynamics of PTEN were produced by the simulation after the dynamics of PIP3 for one cAMP concentration (100 nM) had been used to adjust model parameters. (A), (B), and (C) are simulated responses. (D), (E), and (F) are experimental measurements based on GFP-PTEN analysis in single cells exposed to gradients with a mean cAMP concentration of 1 μmol, 100 nmol, and 10 nmol. See Figure S7 for details on the full dataset of experimental replicates.