Single-Molecule Dynamics Reveals Cooperative Binding-Folding in Protein Recognition
Figure 2
Single-Molecule Fluorescence and Spectroscopy Studies of Cdc42-CBD Protein–Protein Interaction Dynamics
(A) Second-order auto-correlation function, C(t), calculated from a fluorescence intensity trajectory I(t) of a single Cdc42-CBD complex. Solid curve is a bi-exponential fit. (Inset) A single-molecule fluorescence raster-scanning image of GTP-loaded Cdc42 in complex with dye-labeled CBD biosensor.
(B) The occurrence histogram of the single-complex conformational fluctuation rate for 60 individual protein complexes [19].