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Predicting Shine–Dalgarno Sequence Locations Exposes Genome Annotation Errors

Figure 2

Normalized Histogram Plots Showing the RS for the Lowest ΔG ° Values in the TIRs

The x-axis shows the RS, or distance between the 5′ A residue in the rRNA sequence 5′–ACCUCC–3′ from the 3′ tail and the first base in the start codon. Negative numbers indicate that the 5′ A is upstream from the start codon, while positive numbers indicate that it is downstream. The y-axis is the fraction of genes in a genome where the lowest ΔG ° value is at a particular RS.

 Figure 2

doi: https://doi.org/10.1371/journal.pcbi.0020057.g002