The G Protein–Coupled Receptor Subset of the Chicken Genome
Figure 2
Comparison between Curated and Non-Curated Chicken GPCR Sequences
(A) The chart describes the percentage identity between the original Genscan prediction and the manually curated version of the chicken proteins for 158 sequence pairs. The segment labeled 100% contains those proteins that were correctly predicted by Genscan, while the segment labeled 0%–10% contains those pairs that had almost no correctly predicted material.
(B) A histogram describing the percentage identity between 158 human–chicken orthologous pairs as identified from the phylogenetic trees. The solid line and the grey bars represent the comparison between the manually edited chicken proteins and the human orthologs, while the dotted line and the white bars represent the comparison between the human proteins and the non-edited Genscan predictions. The mean percentage identities are 72.9% (standard deviation 14.9) and 56.3% (standard deviation 22.7) for the comparison with the edited and non-edited chicken sequences, respectively. The datasets fit a normal distribution with p = 0.04 and p = 0.08, respectively, using the Kolmogorov–Smirnov test (MiniTab). The lines in the graphs are fitted assuming normal distribution.