Genomic Variability within an Organism Exposes Its Cell Lineage Tree
Figure 5
(A–C) A cell sample lineage tree with a predesigned topology is created by performing single-cell bottlenecks on all the nodes of the tree. Lineage analysis is performed on clones of the root and leaf cells. Three CCTs (A–C) were created using LS174T cells that display MS instability. All topologies were reconstructed precisely. Edge lengths are drawn in proportion to the output of the algorithm. Gray edges represent correct partitions according to the Penny and Hendy tree comparison algorithm [29], and their width represents the bootstrap value [29] (n = 1,000) of the edge. A minimal set of loci yielding perfect reconstruction was found for each CCT (each colored contour represents a different mutation shared by the encircled nodes; see also Figure S2).
(D) There is a linear correlation (R2 = 0.955) between reconstructed and actual node depths.
(E) Reconstruction scores of CCTs A–C using random subsets of MS loci of increasing sizes (average of 500).