A Digital Atlas to Characterize the Mouse Brain Transcriptome
Figure 2
Examples of Gene Expression Patterns
Shown are gene expression patterns revealed by nonradioactive robotic ISH on sagittal sections. Digoxigenin-tagged RNA probes hybridized to cellular mRNA are visualized via a serial amplification that produces a blue-purple signal.
(A) Purkinje cell protein 4 in section 4.
(B) Ly6/neurotoxin 1 in section 6.
(C) 4931408A02Rik in section 9 with inset showing localized expression in midbrain neurons.
(D) A230109K2Rik in section 9 with inset showing localized expression in hypothalamus.
(E) RAS protein-specific guanine nucleotide-releasing factor 1 in section 11.
(F) Gastrin releasing peptide in section 2.
(G) Nephroblastoma overexpressed gene in section 4.
(H) Somatostatin in section 6.