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Open Access
Peer-reviewed
Research Article
Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion
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Carlos Pulido-Quetglas ,
Contributed equally to this work with: Carlos Pulido-Quetglas, Estel Aparicio-Prat, Carme Arnan
Affiliations Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Dr. Aiguader 88, Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain, Institut Hospital del Mar d’Investigacions Mediques (IMIM), Barcelona, Spain
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Estel Aparicio-Prat ,
Contributed equally to this work with: Carlos Pulido-Quetglas, Estel Aparicio-Prat, Carme Arnan
Affiliations Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Dr. Aiguader 88, Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain, Institut Hospital del Mar d’Investigacions Mediques (IMIM), Barcelona, Spain
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Carme Arnan ,
Contributed equally to this work with: Carlos Pulido-Quetglas, Estel Aparicio-Prat, Carme Arnan
Affiliations Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Dr. Aiguader 88, Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain, Institut Hospital del Mar d’Investigacions Mediques (IMIM), Barcelona, Spain
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Taisia Polidori,
Affiliations Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Dr. Aiguader 88, Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain, Institut Hospital del Mar d’Investigacions Mediques (IMIM), Barcelona, Spain
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Toni Hermoso,
Affiliations Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Dr. Aiguader 88, Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain
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Emilio Palumbo,
Affiliations Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Dr. Aiguader 88, Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain, Institut Hospital del Mar d’Investigacions Mediques (IMIM), Barcelona, Spain
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Julia Ponomarenko,
Affiliations Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Dr. Aiguader 88, Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain
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Roderic Guigo,
Affiliations Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Dr. Aiguader 88, Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain, Institut Hospital del Mar d’Investigacions Mediques (IMIM), Barcelona, Spain
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Rory Johnson
* E-mail: rory.johnson@dkf.unibe.ch
Affiliations Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Dr. Aiguader 88, Barcelona, Spain, Universitat Pompeu Fabra (UPF), Barcelona, Spain, Institut Hospital del Mar d’Investigacions Mediques (IMIM), Barcelona, Spain, Department of Clinical Research, University of Bern, Bern, Switzerland, Department of Medical Oncology, Inselspital, University Hospital and University of Bern, Bern, Switzerland
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Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion
- Carlos Pulido-Quetglas,
- Estel Aparicio-Prat,
- Carme Arnan,
- Taisia Polidori,
- Toni Hermoso,
- Emilio Palumbo,
- Julia Ponomarenko,
- Roderic Guigo,
- Rory Johnson
- Published: March 2, 2017
- https://doi.org/10.1371/journal.pcbi.1005341
- See the preprint