Discriminating between negative cooperativity and ligand binding to independent sites using pre-equilibrium properties of binding curves

Federico Sevlever; Juan Pablo Di Bella; Alejandra C. Ventura

doi:10.1371/journal.pcbi.1007929

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Open Access

Peer-reviewed

Research Article

Discriminating between negative cooperativity and ligand binding to independent sites using pre-equilibrium properties of binding curves

Federico Sevlever,

Roles Conceptualization, Data curation, Formal analysis, Investigation, Software, Writing – original draft, Writing – review & editing

Affiliations Department of Physiology, Molecular and Cellular Biology, University of Buenos Aires, Buenos Aires, Argentina, Institute of Physiology, Molecular Biology and Neurosciences, National Research Council (CONICET), Buenos Aires, Argentina

http://orcid.org/0000-0001-6749-4162

⨯
Juan Pablo Di Bella,

Roles Conceptualization, Investigation

Affiliations Department of Physiology, Molecular and Cellular Biology, University of Buenos Aires, Buenos Aires, Argentina, Institute of Physiology, Molecular Biology and Neurosciences, National Research Council (CONICET), Buenos Aires, Argentina
⨯
Alejandra C. Ventura

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Supervision, Writing – original draft, Writing – review & editing

* E-mail: alejvent@fbmc.fcen.uba.ar

Affiliations Department of Physiology, Molecular and Cellular Biology, University of Buenos Aires, Buenos Aires, Argentina, Institute of Physiology, Molecular Biology and Neurosciences, National Research Council (CONICET), Buenos Aires, Argentina

http://orcid.org/0000-0002-5275-3827

⨯

Discriminating between negative cooperativity and ligand binding to independent sites using pre-equilibrium properties of binding curves

Federico Sevlever,
Juan Pablo Di Bella,
Alejandra C. Ventura

Published: June 4, 2020
https://doi.org/10.1371/journal.pcbi.1007929

About the Authors

Federico Sevlever

Roles Conceptualization, Data curation, Formal analysis, Investigation, Software, Writing – original draft, Writing – review & editing

Affiliations Department of Physiology, Molecular and Cellular Biology, University of Buenos Aires, Buenos Aires, Argentina, Institute of Physiology, Molecular Biology and Neurosciences, National Research Council (CONICET), Buenos Aires, Argentina

http://orcid.org/0000-0001-6749-4162

Juan Pablo Di Bella

Roles Conceptualization, Investigation

Alejandra C. Ventura

Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Supervision, Writing – original draft, Writing – review & editing

* E-mail: alejvent@fbmc.fcen.uba.ar

http://orcid.org/0000-0002-5275-3827

Competing Interests

The authors have declared that no competing interests exist.

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Algorithms
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Binding analysis
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Curve fitting
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Simulation and modeling
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Chemical equilibrium
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System stability
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Membrane proteins
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Protein folding
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