In-Vivo Real-Time Control of Protein Expression from Endogenous and Synthetic Gene Networks

Filippo Menolascina; Gianfranco Fiore; Emanuele Orabona; Luca De Stefano; Mike Ferry; Jeff Hasty; Mario di Bernardo; Diego di Bernardo

doi:10.1371/journal.pcbi.1003625

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Open Access

Peer-reviewed

Research Article

In-Vivo Real-Time Control of Protein Expression from Endogenous and Synthetic Gene Networks

Filippo Menolascina ,

Contributed equally to this work with: Filippo Menolascina, Gianfranco Fiore

Current address: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Affiliations TeleThon Institute of Genetics and Medicine (TIGEM), Naples, Italy, Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy
⨯
Gianfranco Fiore ,

Contributed equally to this work with: Filippo Menolascina, Gianfranco Fiore

Affiliations TeleThon Institute of Genetics and Medicine (TIGEM), Naples, Italy, Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy
⨯
Emanuele Orabona,

Affiliation Institute of Microelectronics and Microsystems (IMM), CNR, Naples, Italy
⨯
Luca De Stefano,

Affiliation Institute of Microelectronics and Microsystems (IMM), CNR, Naples, Italy
⨯
Mike Ferry,

Affiliation Department of Bioengineering, University of California, San Diego, La Jolla, California, United States of America
⨯
Jeff Hasty,

Affiliation Department of Bioengineering, University of California, San Diego, La Jolla, California, United States of America
⨯
Mario di Bernardo,

Affiliations Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy, Department of Engineering Mathematics, University of Bristol, Bristol, United Kingdom
⨯
Diego di Bernardo

* E-mail: dibernardo@tigem.it

Affiliations TeleThon Institute of Genetics and Medicine (TIGEM), Naples, Italy, Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy
⨯

In-Vivo Real-Time Control of Protein Expression from Endogenous and Synthetic Gene Networks

Filippo Menolascina,
Gianfranco Fiore,
Emanuele Orabona,
Luca De Stefano,
Mike Ferry,
Jeff Hasty,
Mario di Bernardo,
Diego di Bernardo

Published: May 15, 2014
https://doi.org/10.1371/journal.pcbi.1003625

About the Authors

Filippo Menolascina

Contributed equally to this work with: Filippo Menolascina, Gianfranco Fiore

Current address: Department of Civil and Environmental Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America

Affiliations TeleThon Institute of Genetics and Medicine (TIGEM), Naples, Italy, Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy

Gianfranco Fiore

Contributed equally to this work with: Filippo Menolascina, Gianfranco Fiore

Affiliations TeleThon Institute of Genetics and Medicine (TIGEM), Naples, Italy, Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy

Emanuele Orabona

Affiliation Institute of Microelectronics and Microsystems (IMM), CNR, Naples, Italy

Luca De Stefano

Affiliation Institute of Microelectronics and Microsystems (IMM), CNR, Naples, Italy

Mike Ferry

Affiliation Department of Bioengineering, University of California, San Diego, La Jolla, California, United States of America

Jeff Hasty

Affiliation Department of Bioengineering, University of California, San Diego, La Jolla, California, United States of America

Mario di Bernardo

Affiliations Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy, Department of Engineering Mathematics, University of Bristol, Bristol, United Kingdom

Diego di Bernardo

* E-mail: dibernardo@tigem.it

Affiliations TeleThon Institute of Genetics and Medicine (TIGEM), Naples, Italy, Department of Electrical Engineering and Information Technology, University of Naples Federico II, Naples, Italy

Competing Interests

The authors have declared that no competing interests exist.

Author Contributions

Conceived and designed the experiments: FM GF DdB MdB. Performed the experiments: FM GF. Analyzed the data: FM GF. Contributed reagents/materials/analysis tools: EO LDS FM GF. Wrote the paper: FM GF DdB MdB. Technology transfer: MF JH.

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